California State University Dissociation Reaction of Na3 Po4 in Water Lab Report

Question Description

(3)For our purpose, assume that you are detecting Na3PO4 in water. Please write the dissociationreaction of Na3PO4 in water. What type of acid/base system is this:

(a)strong/weak,

(b)acid or base,

(c)monoprotic/polyprotic? If polyprotic, how many protons?

(4)Assume you transfer 50 mL of that sample and you will titrate it with 2 mmol/L standardized solutionof strong acid/base. Please propose what acid or what base you will use as titrant for this titration.Explain your decision.

(5)Calculate the Equivalence volume (or volumes) and the pH at the (final) equivalence point.

(6)Calculate the pH after you added 4 mL of titrant to your sample.

(7)For this titration you will need an indicator. Please look at this list and choose the indicator to detectthe (final) equivalence point. Explain your choice.

(7)Phosphates are used in your body to keep the pH constant at 7.4. What are the principal species ofphosphates that exist at this pH? What is the concentration of these species at pH 7.4? [hint: you willneed to use the total concentration of phosphates that you measured with random number generator]

(8)Human blood contains about 9 g/L of sodium chloride. Calculate the activity of phosphate in yoursample.

(9)The healthy level of phosphate in blood is between 0.87 and 1.45 mmol/L and is related to thecalcium level. To measure the level of phosphate you need to bind the calcium before you start thetitration. The normal level of “free” calcium in blood is 4.4-5.2 mg/dL. Calculate how much EDTA youshould use to bind all the calcium before your phosphate titration. [Hint: your sample is still 50 mL]

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