Northlake College Electrophoretic Separation of Proteins Research

Question Description

report needs to include basic protocols, instrumentation, reagents, and types of research that utilizes 2D SDS-PAGE, areas you could focus on are listed below. Develop a work flow when identifying proteins in a complex mixture. Issues that may arise during the PAGE run and how would you would trouble shoot and rectify it. You may want to contact some of the company’s technical support lines and ask specific questions about issues with 2D. Do not use Wikipedia as it is flawed with many mistakes. Do not limit your report to just the few items above, look into some of the research that is currently being done using this technique, you could add information about how proteins are identified using MS.

Suggested focus areas, if you find something different that you are interested in, go for it.

Extraction Techniques and Applications: Biological/Medical/Environmental and Forensics.
Genetic Heterogeneity of Human Brain Proteins and their Diagnosis in Living Patients
Nitric Oxide, Part G Oxidative and Nitrosative Stress in Redox Regulation of Cell Signaling
Proteomics in Biomedicine and Pharmacology
PRIONS
Proteins in Pesticide Technology
Heterologous Expression of Proteins in Trichoderma

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