Activity 1: Biuret Assay for Protein

Table 1: Biuret Assay for Protein

	Your Prediction	Actual Assay Results Description	Protein Present? (Yes/No)	Nutrition Label Information
PositiveControl (BSA)		Purple
Negative Control (Water)		Light blue
Milk		purple
Apple Juice		Light blue
Potato flakes		Light blue
Gelatin		purple
Egg White		purple

Notes:

Activity 2:Lugol’s Iodine Test for Starch

Table 2: Lugol’s Test for Starch

	Prediction	Assay Results Description	Starch Present? (Yes/No)	Nutrition Label Information
Positive Control (1% corn starch)		Dark Blue/black
Negative Control (Water)		golden
Milk		golden
Apple Juice		golden
Potato flakes		Dark blue/black
Gelatin		golden
Egg White		golden

Notes:

Activity 3: Benedict’s Test for Reducing Sugars

Table 3: Benedicts Test for Reducing Sugars

	Prediction	Assay Results Description	Sugar Present? (Yes/No)	Nutrition Label Information
Positive Control (10% Glucose)		Brick red color
Negative Control (Water)		Light blue
Milk		Orange-red color
Apple Juice		Orange-red color
Potato flakes		Yellow-green
Gelatin		Light blue
Egg White		Light blue

Notes:

Activity 4: Paper Test for Lipids

Table 4: Paper Test for Lipids

	Prediction	Assay Results Description	Lipids Present? (Yes/No)	Nutrition Label Information
Positive Control (Corn Oil)		Greasy spot
Negative Control (Water)		No spot
Milk		Slightly greasy spot
Apple Juice		No spot
Potato flakes		No spot
Gelatin		No spot
Egg White		No spot

Notes:

Activity 5: Summary of Results

Table 5: Summary of Biological Molecules in Foods

	Biuret test for Protein	Lugol’s test for starch	Benedicts test for reducing sugars	Paper test for lipids
Milk
Apple Juice
Potato flakes
Gelatin
Egg White

Activity 6: Communicating Your Results

In the space below, summarize the results of your tests. Did your results match your predictions? Were there any cases where you were surprised at the results you obtained? How did your results compare to the information from the nutritional data sheets?

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