Grossmont College Biuret Assay for Protein Case Study
Question Description
Activity 1: Biuret Assay for Protein
Table 1: Biuret Assay for Protein
Your Prediction |
Actual Assay Results Description |
Protein Present? (Yes/No) |
Nutrition Label Information |
|
PositiveControl (BSA) |
Purple |
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Negative Control (Water) |
Light blue |
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Milk |
purple |
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Apple Juice |
Light blue |
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Potato flakes |
Light blue |
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Gelatin |
purple |
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Egg White |
purple |
Notes:
Activity 2:Lugols Iodine Test for Starch
Table 2: Lugols Test for Starch
Prediction |
Assay Results Description |
Starch Present? (Yes/No) |
Nutrition Label Information |
|
Positive Control (1% corn starch) |
Dark Blue/black |
|||
Negative Control (Water) |
golden |
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Milk |
golden |
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Apple Juice |
golden |
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Potato flakes |
Dark blue/black |
|||
Gelatin |
golden |
|||
Egg White |
golden |
Notes:
Activity 3: Benedicts Test for Reducing Sugars
Table 3: Benedicts Test for Reducing Sugars
Prediction |
Assay Results Description |
Sugar Present? (Yes/No) |
Nutrition Label Information |
|
Positive Control (10% Glucose) |
Brick red color |
|||
Negative Control (Water) |
Light blue |
|||
Milk |
Orange-red color |
|||
Apple Juice |
Orange-red color |
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Potato flakes |
Yellow-green |
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Gelatin |
Light blue |
|||
Egg White |
Light blue |
Notes:
Activity 4: Paper Test for Lipids
Table 4: Paper Test for Lipids
Prediction |
Assay Results Description |
Lipids Present? (Yes/No) |
Nutrition Label Information |
|
Positive Control (Corn Oil) |
Greasy spot |
|||
Negative Control (Water) |
No spot |
|||
Milk |
Slightly greasy spot |
|||
Apple Juice |
No spot |
|||
Potato flakes |
No spot |
|||
Gelatin |
No spot |
|||
Egg White |
No spot |
Notes:
Activity 5: Summary of Results
Table 5: Summary of Biological Molecules in Foods
|
Biuret test for Protein |
Lugols test for starch |
Benedicts test for reducing sugars |
Paper test for lipids |
Milk
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Apple Juice
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Potato flakes
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Gelatin
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Egg White
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Activity 6: Communicating Your Results
In the space below, summarize the results of your tests. Did your results match your predictions? Were there any cases where you were surprised at the results you obtained? How did your results compare to the information from the nutritional data sheets?
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