Texas Womans PCR Data Through Gel Electrophoresis Experimentation Lab Report

Question Description

The data and template required are attached. Please use the given data and template. See the instruction links added below.

https://unt.instruc ture.com/courses/32575/files/88…

https://unt.instructure.com/courses/32575/files/88…

these are 2 links of the notes provided by my instructor.

Use this template to sketch your predictions: Gel Template.jpg

Example of a gel: GMO PCR Data for report.png

Experimental Design must include:

Identify the independent variable and dependent variable in this experiment. (2 points)
Choose a food of interest and write an acting hypothesis for this experiment. (2 points)
- This hypothesis should include the predicted cause and effect (the if ___, then _____) as well as the reason for that prediction (because ________). A hypothesis can be multiple sentences and does not need to follow the “if-then-because” format so long as it communicates the necessary information. See tips on hypothesis writing here: Hypothesis-Writing Resources.docx
Annotated gel figure with description (4 points)
- Label each lane with its contents. Each of these should be depicted in a lane:
  - standard ladder
  - positive GMO control
  - negative GMO control
  - positive Plant control
  - negative Plant control
  - your prediction for the experimental food with GMO primer and with Plant primer

Discussion Points (3 points each):

1. Explain the purpose of the standard ladder in gel electrophoresis.
2. Explain the purpose of positive and negative controls. Why do we use both?
3. Why would we use two different primers in this experiment? Can we trust a result from a test using only one sample amplified with the GMO primers (as opposed to two samples, one amplified with GMO primers and one amplified with Plant primers)?
4. This experiment is extremely similar to COVID-19 PCR tests. What is the difference between this GMO PCR test and the COVID-19 PCR test? (Cite outside sources if needed)

*For all discussion points, use the data to support your conclusions. One-sentence answers will not be considered a discussion and will not receive full credit.*



^{Have a similar assignment? "Place an order for your assignment and have exceptional work written by our team of experts, guaranteeing you A results."}

Our Service Charter

1. Professional & Expert Writers: Eminence Papers only hires the best. Our writers are specially selected and recruited, after which they undergo further training to perfect their skills for specialization purposes. Moreover, our writers are holders of masters and Ph.D. degrees. They have impressive academic records, besides being native English speakers.

2. Top Quality Papers: Our customers are always guaranteed of papers that exceed their expectations. All our writers have +5 years of experience. This implies that all papers are written by individuals who are experts in their fields. In addition, the quality team reviews all the papers before sending them to the customers.

3. Plagiarism-Free Papers: All papers provided by Eminence Papers are written from scratch. Appropriate referencing and citation of key information are followed. Plagiarism checkers are used by the Quality assurance team and our editors just to double-check that there are no instances of plagiarism.

4. Timely Delivery: Time wasted is equivalent to a failed dedication and commitment. Eminence Papers are known for the timely delivery of any pending customer orders. Customers are well informed of the progress of their papers to ensure they keep track of what the writer is providing before the final draft is sent for grading.

5. Affordable Prices: Our prices are fairly structured to fit in all groups. Any customer willing to place their assignments with us can do so at very affordable prices. In addition, our customers enjoy regular discounts and bonuses.

6. 24/7 Customer Support: At Eminence Papers, we have put in place a team of experts who answer all customer inquiries promptly. The best part is the ever-availability of the team. Customers can make inquiries anytime.